Beilstein J. Org. Chem.2018,14, 2665–2679, doi:10.3762/bjoc.14.244
) and CHO-β (FR +ve) cell lines and their respective competition experiments demonstrate the specificity of the newly synthesized bioconstructs for future application in fluorescent guided intra-operative imaging.
Keywords: chelating linker; confocal studies; continuoussyntheticprocess; fluorescent
polypeptide chains 9a (Pg = Mtt) and 9b (Pg = Mmt) are generally cleaved under acidic conditions. Therefore, it becomes pertinent to analyze the stability of the chlorotrityl resin and ε-amino protecting groups in the polypeptide chains 9a and 9b to achieve our multiple objectives in a continuoussynthetic
process without the isolation of any of the intermediates.
The peptide chain cleavage conditions for chlorotrityl resin are well established and the ε-amino trityl protecting groups of Fmoc-Lys-(Mtt or Mmt)-OH of the side chain are usually acid-labile with an order of stability: Trt (chlorotrityl) > Mtt
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Graphical Abstract
Figure 1:
(a) Structure of universal nova tag resin, (b) structure of H-L-Cys(Trt)-2-ClTrt resin.